Total coliforms / Escherichia coli / Clostridium perfringens / Enterococci / Pseudomonas aeruginosa / Colony Counts (22ºC/3 days) and (37ºC/2 day) in Bottled Water Proficiency Test

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Product Specification

Proficiency Test Information
Product Code Proficiency Test Matrix Approx. Size


Water (Bottled)

3 x lyophilised sample


Total Coliforms, Escherichia coli, Clostridium perfringens, Enterococci, Pseudomonas aeruginosa, Colony Counts (22°C/3 days), Colony Counts (37°C/2 days)


Enumeration Test

Test Description

Bottled water is a lifestyle choice for millions of consumers every day and also provides a safe source of drinking water when microbial or chemical contamination of the water distribution system occurs (or such infrastructure is simply not present). It is therefore essential that the analytical laboratory testing commercially produced water can accurately enumerate the indicator organisms that may be present in the water in line with the regulations governing the safety of bottled water.

This proficiency test brings together the seven essential tests for the quality of bottled water in one proficiency test in order to present a realistic testing scenario for analytical laboratories and provide excellent value for money. The test is provided as three glass vials with volume of 2 ml to be rehydrated and diluted to 1 litre in accordance with FAPAS sample preparation instructions. Participants must submit their results as cfu/ml for Colony Counts (22°C/72 hr) and (37°C/48 hr), as cfu/50 ml for Clostridium perfringens and as cfu/250 ml for Total coliforms, Escherichia coli, Enterococci and Pseudomonas aeruginosa.

Colony counts at 22°C and 37°C are long established tests for the overall hygienic status of the water and the Enumeration of coliforms and Escherichia coli, are designed to indicate potential faecal contamination and possible contamination with pathogens. Enterococci and Clostridium perfringens area also used as indicators of previous faecal contamination as they are both potentially capable of surviving for longer periods than coliforms. 

Pseudomonas aeruginosa is an environmental organism which can grow in waters with minimal levels of nutrients and cause off tastes and odours. It is also an opportunistic pathogen but it is not normally a problem for healthy individuals.

Please NOTE: This test is designed for analysis using membrane filtration techniques, and therefore results reported as MPN (Most Probable Number) will not be accepted.

This product is suitable for routine laboratory performance check as well as verification of standardised method or validation of a new laboratory method.

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