Cell Count by Flow Cytometry in Drinking Water Proficiency Test

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FWM13-DRW1 | Drinking Water | Cell Count by Flow Cytometry

Product Specification

Proficiency Test Information
Product Code Proficiency Test Matrix Approx. Size
FWM13-DRW1

DW106m06

Water

10 ml

Analytes

Cell count by flow cytometry

Comments

Cell count by flow cytometry is not currently within scope of accreditation to ISO 17043

Test Description

Microbiological monitoring in support of drinking water quality assurance covers a range of indicator organisms. Fapas drinking water microbiology proficiency tests are enumeration and identification tests which cover the major indicators of colony counts, total coliforms (with a separate E. coli test), enterococci, Pseudomonas and Clostridium

Colony counts are traditionally used to determine the hygienic quality of potable waters, but these come with limitations in terms of the time taken to carry out the test and the types of bacteria which can be enumerated using the cultural methods.

Flow cytometry (FCM) provides a method for direct, real-time measurement of bacterial cells in water, with the method most likely to be used to enumerate bacteria in final treated waters for distribution to consumers. The method can differentiate between viable and non-viable cells with the application of different nucleic acid stains, and thereby give more information than traditional methods such as the heterotrophic plate count.

Proficiency testing provides a means to monitor laboratory results by comparing data between laboratories using technically equivalent methods. Participation in this PT allows laboratories to be compared with their peers using FCM methods, which are not readily comparable with the traditional methods of measuring water quality.

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